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KMID : 0380420010250040253
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Journal of Prventive Veterinary Medicine
2001 Volume.25 No. 4 p.253 ~ p.256
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Determination of Anionic Surfactants in Raw Milks by Methylene Blue Colorimetry
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이희수/Lee, Hee Soo
이명헌/정갑수/조병훈/이광직/이승환/김정임/Lee, Myoung Heon/Chung, Gab Soo/Cho, Byung Hoon/Lee, Kwang Jick/Lee, Sung Hwan/Kim, Chung Lim
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Abstract
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A determination method for anionic surfactants residue in raw milk was established using methylene blue method. The residue in raw milk was first precipitated with acetonitril, seperated by centrifugation, concentrated by evaporation of supernatant, dissolved with distilled water, allowed to complex formation by adding the methylene blue and extracted with chloroform. The final was analysed and quantified by spectrophotometry.
Based on the collaborative studies in 3 laboratories after spiking 5 to 20 ppm of standard solution, the mean of recovery rate within laboratory repeatability were 85.9%, 104.6% and 94.9%, respectively and the coefficient of variation were 9.3%, 9.8% and 6.4%. In addition, the coefficient of variation between laboratory repeatability was 16.1% which would be acceptable to the recommended guideline of CODEX.
In conclusion, the present method makes feasibilities for quantified and qualified determination of anionic surfactants residue in raw milk.
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